Bioimaging – GIMM Bioimaging – GIMM
↑

Bioimaging

Bioimaging

Back to Scientific and Clinical Platforms ⇾

The Bioimaging Unit offers state-of-the-art personalized support for all research done with Light Microscopy at GIMM, housing over 30 high-end systems including super-resolution, point-scanning confocal, spinning disk confocal, multiphoton, lightsheet, live cell imaging, high-content screening, slide scanning, and advanced image analysis workstations. 

From the very first question to the moment of discovery and publication, we sit with researchers to advise, teach, train, and assist in image acquisition, analysis and processing, empowering scientists with cutting-edge technology and expertise.

Together with continuous hands-on training of users, we organize regular courses and workshops in basic and advanced light microscopy techniques and bioimage analysis tools. 

Bioimaging info

Spinning Disk Confocal Microscopes

  • 3i Marianas SDC (Oeiras) – inverted microscope with motorized stage, piezo, stage top incubation, focus drift correction, sCMOS camera, and photomanipulation (FRAP + ablation) module.
  • 3i Marianas SDC (Lisbon) – inverted microscope with motorized stage, piezo, stage top and large cage incubation, and EM-CCD camera.
  • 3i Marianas SDC II + TIRF BSL2 (Oeiras) – inverted microscope with motorized stage, piezo, stage top incubation, focus drift correction, sCMOS camera, photomanipulation (FRAP) module, super-resolution, and TIRF, in a BSL2 room.
  • Andor Dragonfly SDC (Oeiras) – inverted microscope with motorized stage, piezo, stage top incubation, focus drift correction, and sCMOS camera.
  • Andor Spinning Disk W1 (Oeiras) – inverted microscope with motorized stage, piezo, stage top incubation, focus drift correction, and sCMOS and EM-CCD cameras.
  • Leica Spinning Disk Confocal (Oeiras) – inverted microscope with motorized stage, piezo, stage top incubation, EM-CCD camera, and photomanipulation module.
  • Zeiss Cell Observer SD (Lisbon) – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and EM-CCD camera.

 

Point Scanning Confocal Microscopes

  • Zeiss LSM 880 (Lisbon) – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and super-resolution.
  • Zeiss LSM 900 (Oeiras) – inverted microscope with motorized stage, piezo, stage top and large cage incubation, super-resolution, and AI sample finder.
  • Zeiss LSM 980 (Lisbon) – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and super-resolution.
  • Zeiss LSM 980 (Oeiras) – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and super-resolution.
  • Zeiss LSM 980-32 (Lisbon) – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, super-resolution, spectral imaging, AI sample finder, and auto-immersion.

Automated Point Scanning Confocal Microscope

  • Zeiss Celldiscoverer 7 with LSM 900 (Lisbon) – fully automated inverted microscope with incubation, focus drift correction, super-resolution, confocal and widefield imaging modes, ideal for automated live imaging in well-plates and Petri dishes.

Multiphoton Microscopes

  • Leica SP8 MP (Lisbon) – upright microscope with dual pulsed infra-red laser, motorized stage, large dark cage, heated pad, and volatile anaesthesia system.
  • Prairie Multiphoton (Oeiras) – upright microscope with pulsed infra-red laser, motorized heated stage, and volatile anaesthesia system.

Light Sheet Fluorescence Microscopes

  • Miltenyi UltraMicroscope Blaze (Oeiras) – light sheet microscope prepared for clearing media immersion imaging, with sCMOS camera.
  • OPT SPIM (Oeiras) – mesoscope built in-house for optical projection tomography and light sheet imaging of large samples.
  • Zeiss Lightsheet Z.1 (Lisbon) – light sheet microscope prepared for water and clearing media immersion imaging, with chamber incubation and sCMOS camera.
  • Zeiss Lightsheet Z.1 (Oeiras) – light sheet microscope prepared for water immersion imaging, with chamber incubation and two sCMOS cameras.

Super-Resolution Single Molecule Fluorescence Microscope

  • ONI Nanoimager (Oeiras) – bench top inverted super-resolution microscope optimized for single molecule localization (dSTORM, PALM, PAINT, single-particle tracking, smFRET, HILO, TIRF) with incubation, focus drift correction, and sCMOS camera.

Slide Scanner

  • Zeiss Axioscan 7 (Lisbon) – automated slide scanner for fluorescence and brightfield, with monochromatic and color CMOS cameras and automated scanning of up to 100 slides per run.

 

Widefield Fluorescence Microscopes

  • Agilent Cytation C5 (Oeiras) – inverted automated imaging multimode reader with incubation and CMOS camera.
  • DeltaVision OMX-SIM (Oeiras) – inverted structured illumination (super-resolution) and widefield microscope with motorized stage, incubation, two sCMOS cameras, and SIM-TIRF.
  • Nikon Eclipse Ti (Lisbon) – inverted microscope with motorized stage, stage top incubation, focus drift correction, sCMOS camera, calcium and contractibility system with high-speed CMOS camera, high-speed PMT, and electrical field stimulation.
  • Nikon HTM-HCS (Oeiras) – inverted microscope with motorized stage, stage top and large cage incubation, focus drift correction, DIC, phase contrast, and sCMOS camera.
  • Zeiss Axiovert 200M (Lisbon) – inverted microscope with motorized stage, DIC, and CCD camera.
  • Zeiss Cell Observer (Lisbon) – inverted microscope with motorized stage, stage top and large cage incubation, focus drift correction, DIC, Phase contrast, monochromatic CMOS and sCMOS cameras, and AKOYA Phenocycler fluidics system for automated staining and imaging of whole slide tissues for Spatial Biology applications.
  • Zeiss Imager with Apotome 2 (Oeiras) – upright microscope with motorized stage with 8 slide carrier, DIC, color CMOS camera, monochromatic sCMOS camera, and structured illumination for optical sectioning of fluorescent samples. 

Fluorescence Stereo Microscopes

  • Zeiss Axiozoom.V16 (Lisbon) – motorized stereo microscope with a monochromatic CCD camera.
  • Zeiss Stereo Lumar.V12 (Oeiras) – motorized stereo microscope with color CCD or monochromatic sCMOS camera.

Brightfield Microscopes

  • Leica DM2500 (Lisbon) – upright microscope with DIC, phase contrast, polarization, and a color CCD camera.
  • Nikon PhaCo (Oeiras) – upright microscope with phase contrast.

Other Imaging Systems

  • IVIS Lumina (Lisbon) – bioluminescence / fluorescence imaging system.

Workstations

  • Amira Workstation (Oeiras) – image processing and analysis, with Amira.
  • Chronos (Lisbon) – image processing and analysis with ZEN Desk, and Imaris.
  • Huygens Workstation (Oeiras) – image processing and analysis, with Huygens. 
  • Imaris Workstation (Oeiras) – image processing and analysis, with Imaris.
  • Z2 Workstation (Oeiras) – image processing and analysis, with ZEN.
  • Z3 Workstation (Oeiras) – image processing and analysis, with ZEN.
  • Zeiss LSM 980-32 Workstation (Lisbon) – image processing and analysis, with ZEN and Arivis Pro.

If you used our services, please acknowledge us in publications or other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples:

Materials and Methods: “Microscopy experiments were performed at the Bioimaging Unit of the Gulbenkian Institute for Molecular Medicine.”

Acknowledgements: “We thank the technical support of the Bioimaging Unit of the Gulbenkian Institute for Molecular Medicine, funded by PPBI-POCI-01-0145-FEDER-022122.”

Thank you!

The Bioimaging Unit serves GIMM researchers, as well as external academic and industrial users. All users must be registered in Agendo, our online booking system.

Please contact us (joserino@medicina.ulisboa.pt) for more information.