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Bioimaging

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The Bioimaging Platform offers state-of-the-art personalized support in all steps of research done with Light Microscopy at GIMM, housing over 30 high-end systems including point-scanning confocal, spinning disk confocal, multiphoton, light sheet, widefield, slide scanning, and advanced image analysis workstations.

From the very first question to the moment of discovery and publication, we sit with researchers to advise, teach, train, and assist in image acquisition, analysis and processing, empowering scientists with cutting-edge technology and expertise.

In addition to daily hands-on training of users, we organize regular courses and workshops in basic and advanced light microscopy techniques and bioimage analysis.

We are a node of Portuguese Platform of Bioimaging (PPBI), a nationwide consortium of imaging facilities which integrates the European research infrastructure Euro-BioImaging ERIC. We are also a ZEISS labs@location Partner, integrating a community of advanced microscopy facilities providing in-depth knowledge, dedicated services across a wide range of applications and instrumentation, and scientific support to ZEISS customers.

For more detailed information about the Platform, please visit the Bioimaging Wiki.

Bioimaging info

The Bioimaging Platform provides training and assistance in planning optical microscopy-oriented projects, choosing materials and equipment, preparing samples, acquiring data, analyzing and processing acquired images, presenting and publishing results.

The Bioimaging systems are available on the online booking system for trained researchers 7 days a week and 24 hours per day. User training in each system is mandatory, even for researchers with previous microscopy experience.

For more detailed information on how to use our Services, please visit the Bioimaging Wiki.

If you used our services, please acknowledge us in publications or other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples:

Materials and Methods: “Microscopy experiments were performed at the Bioimaging Platform of the Gulbenkian Institute for Molecular Medicine.”

Acknowledgements: “We thank the technical support of the Bioimaging Platform of the Gulbenkian Institute for Molecular Medicine, funded by PPBI-POCI-01-0145-FEDER-022122.”

Thank you!

Widefield Systems

[Oeiras] Agilent Cytation C5 – inverted automated imaging multimode reader with incubation and CMOS camera.
[Oeiras] DeltaVision OMX-SIM – inverted structured illumination and widefield microscope with motorized stage, incubation, two sCMOS cameras, and TIRF.
[Lisbon] Nikon Eclipse Ti – inverted microscope with motorized stage, stage top incubation, focus drift correction, sCMOS camera, calcium and contractibility system with high-speed CMOS camera, high-speed PMT, and electrical field stimulation.
[Oeiras] Nikon HTM-HCS – inverted microscope with motorized stage, stage top and large cage incubation, focus drift correction, DIC, and sCMOS camera.
[Oeiras] ZEISS Axio Imager with Apotome 2 – upright microscope with motorized stage with 8 slide positions, DIC, color CMOS camera, monochromatic sCMOS camera, and structured illumination for optical sectioning of fluorescent samples.
[Lisbon] ZEISS Axioscan 7 – automated slide scanner for fluorescence and brightfield, with monochromatic and color CMOS cameras and automated scanning of up to 100 slides per run.
[Lisbon] ZEISS Axiovert 200M – inverted microscope with motorized stage, DIC, and CCD camera.
[Lisbon] ZEISS Cell Observer – inverted microscope with motorized stage, stage top and large cage incubation, focus drift correction, DIC, Phase contrast, monochromatic CMOS and sCMOS cameras, and an AKOYA Phenocycler fluidics system for automated staining and imaging of whole slide tissues for Spatial Biology applications.

Spinning Disk Confocals

[Lisbon] 3i Marianas SDC – inverted microscope with motorized stage, piezo, stage top and large cage incubation, and EM-CCD camera.
[Oeiras] 3i Marianas SDC – inverted microscope with motorized stage, piezo, stage top incubation, focus drift correction, sCMOS camera, and photomanipulation module.
[Oeiras] 3i Marianas SDC II + TIRF (BSL2) – inverted microscope with motorized stage, piezo, stage top incubation, focus drift correction, sCMOS camera, photomanipulation module, super-resolution, and TIRF, in a BSL2 room.
[Oeiras] Andor Dragonfly SDC – inverted microscope with motorized stage, piezo, stage top incubation, focus drift correction, and sCMOS camera.
[Oeiras] Leica Spinning Disk Confocal – inverted microscope with motorized stage, piezo, EM-CCD camera, and photomanipulation module.
[Lisbon] ZEISS Cell Observer SD – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and EM-CCD camera.

Point Scanning Confocals

[Lisbon] ZEISS Celldiscoverer 7 with LSM 900 – fully automated inverted microscope with incubation, focus drift correction, widefield and confocal imaging modes (with Airyscan 2 for super-resolution), ideal for automated live imaging in well-plates and Petri dishes. Equipped with a PAA KX-2 robotic arm, an Agilent Bravo automatic dispenser and a Cytomat C2 incubator for high-throughput acquisition.
[Lisbon] ZEISS LSM 880 with Airyscan – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and super-resolution.
[Oeiras] ZEISS LSM 900 with Airyscan 2 – inverted microscope with motorized stage, piezo, stage top and large cage incubation, and super-resolution.
[Lisbon] ZEISS LSM 980 with Airyscan 2 – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and super-resolution.
[Oeiras] ZEISS LSM 980 with Airyscan 2 – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, and super-resolution.
[Lisbon] ZEISS LSM 980-32 with Airyscan 2 – inverted microscope with motorized stage, piezo, stage top and large cage incubation, focus drift correction, super-resolution, spectral imaging, AI sample finder, and water auto-immersion.

Light Sheet Microscopes

[Oeiras] Miltenyi UltraMicroscope Blaze – light sheet microscope prepared for clearing media immersion imaging, with sCMOS camera.
[Oeiras] Mesoscope (SPIM+OPT) – mesoscope for optical projection tomography and light sheet imaging of large samples.
[Lisbon] ZEISS Lightsheet Z.1 – light sheet microscope prepared for water and clearing media immersion imaging, with chamber incubation and sCMOS camera.
[Oeiras] ZEISS Lightsheet Z.1 – light sheet microscope prepared for water immersion imaging, with chamber incubation and two sCMOS cameras.

Brightfield / Stereo Microscopes

[Lisbon] Leica DM2500 – upright microscope with DIC, Phase Contrast, Polarization, and a color CCD camera.
[Oeiras] ZEISS Stereo Lumar.V12 – motorized stereo microscope with color CCD and monochromatic sCMOS cameras.
[Lisbon] ZEISS Axio Zoom.V16 – motorized stereo microscope with a monochromatic CCD camera.

Multiphoton Microscopes

[Lisbon] Leica SP8 MP – upright microscope with dual pulsed infra-red laser, motorized stage, large dark cage, heated pad, and volatile anaesthesia system.
[Oeiras] Prairie Multiphoton – upright microscope with pulsed infra-red laser, motorized stage, large dark cage, and heated stage.

Bioluminescence System

[Lisbon] IVIS Lumina – bioluminescence / fluorescence imaging system.

Workstations

[Oeiras] Amira Workstation – image processing and analysis, with Amira.
[Lisbon] Chronos Workstation – image processing and analysis with Imaris, and ZEN.
[Oeiras] Huygens Workstation – image processing and analysis, with Huygens.
[Oeiras] Imaris Workstation – image processing and analysis, with Imaris.
[Oeiras] Z2 Lightsheet Workstation – image processing and analysis, with Arivis.
[Oeiras] Z3 Big Data Workstation – image processing and analysis, with Arivis and ZEN.